Modeling methanogenesis with a genome‐scale metabolic reconstruction of Methanosarcina barkeri
نویسندگان
چکیده
منابع مشابه
Modeling methanogenesis with a genome-scale metabolic reconstruction of Methanosarcina barkeri
We present a genome-scale metabolic model for the archaeal methanogen Methanosarcina barkeri. We characterize the metabolic network and compare it to reconstructions from the prokaryotic, eukaryotic and archaeal domains. Using the model in conjunction with constraint-based methods, we simulate the metabolic fluxes and resulting phenotypes induced by different environmental and genetic condition...
متن کاملBioenergetics of methanogenesis from acetate by Methanosarcina barkeri.
Methane formation from acetate by resting cells of Methanosarcina barkeri was accompanied by an increase in the intracellular ATP content from 0.9 to 4.0 nmol/mg of protein. Correspondingly, the proton motive force increased to a steady-state level of -120 mV. The transmembrane pH gradient however, was reversed under these conditions and amounted to +20 mV. The addition of the protonophore 3,5,...
متن کاملInhibition of methanogenesis by interaction of aluminium ion with co-factor, F-420, in Methanosarcina barkeri.
Methane emission was inhibited by aluminium ion in paddy fields. Addition of Al3+ (20 mM) to the culture medium containing cells of pure Methanosarcina barkeri, inhibited methanogenesis. Methanogenic co-factor, F-420, was isolated and purified from Methanosarcina barkeri MS. Spectrophotometric and spectrofluorometric analysis of interaction between co-factor, F-420, and Al3+ revealed that they ...
متن کاملMethanogenesis from Choline by a Coculture of Desulfovibrio sp. and Methanosarcina barkeri.
A sulfate-reducing vibrio was isolated from a methanogenic enrichment with choline as the sole added organic substrate. This organism was identified as a member of the genus Desulfovibrio and was designated Desulfovibrio strain G1. In a defined medium devoid of sulfate, a pure culture of Desulfovibrio strain G1 fermented choline to trimethylamine, acetate, and ethanol. In the presence of sulfat...
متن کاملUncoupling of Methanogenesis from Growth of Methanosarcina barkeri by Phosphate Limitation.
Production of methane by Methanosarcina barkeri from H(2)-CO(2) was studied in fed-batch culture under phosphate-limiting conditions. A transition in the kinetics of methanogenesis from an exponentially increasing rate to a constant rate was due to depletion of phosphate from the medium. The period of exponentially increasing rate of methanogenesis was extended by increasing the initial concent...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Systems Biology
سال: 2006
ISSN: 1744-4292,1744-4292
DOI: 10.1038/msb4100046